Supavadee Boontha is a Lecturer at School of Pharmaceutical Sciences at University of Phayao. She studied B. Pharm at the Naresuan University, and also received her Ph.D. in Pharmaceutical technology from Faculty of Pharmaceutical Sciences at the Naresuan University, working on vaccine delivery and delivery system of herbal medicine for cancer treatment.\r\n
This study were aimed to determine cytotoxic and anti-migration effects of Morinda citrifolia L. fruit extract on human breast cancer cells and to prepare microemulsions for the extract. The ethanolic Morinda citrifolia L. fruit extract showed cytotoxicity with IC50 values of 220.0 ±15.0 µg/ml and anti-migration effect on human MCF-7 cancer cells with significant effect at 100 µg/ml. Microemulsion (ME) systems were developed by titration method and further selected to incorporate the extract based on the suitable physicochemical characteristics of the ME prepared. The ME systems consisted of olive oil or isopropyl myristate as an oil phase, PEG40 hydrogenated castor oil or Tween 80 as a surfactant and Span 80 as a co-surfactant. The developed ME containing the extract were evaluated regarding their physical appearance, viscosity and pH before and after stability test. The stability study was carried out at room temperature and 45 oC for 60 days. After stability test at different temperatures, the ME containing the extract consisting of 56% w/w olive oil, 20% w/w tween 80, 20% w/w span 80, 0.7% w/w the extract, and 4% w/w water phase was shown to be the most attractive ME for treatment breast cancer via topical route.
Benjaporn Buranrat is a Asst. Prof. at the Faculty of Medicine at the Mahasarakham University. She studied Public Health Science at the Khon Kaen University, and received her PhD in Pharmacology from Faculty of Medicine at the Khon Kaen University, working on mevalonate pathway inhibitors and cancer. She did her postdoctoral work at Penn State University, USA, working on ferritin effects and MCF-7 cancer cells. She has authored or co-authored over 15 manuscripts. Her laboratory has worked in cancer cells death, cell apoptosis, mevalonate pathway.
In this study we examined how Oroxylum indicum fruit extracts can be affected the viability and migration of the human breast cancer cells and the mechanism of actions as well. The results, O. indicum extract strongly induced MCF-7 cell death in a dose- and time-dependent manner, with IC50 values of 131.3 + 19.2 ug/mL at 48 h. Moreover, O. indicum extracts also caused a dose-dependent decrease in colony forming ability with IC50 values of 51.46 + 6.43 ug/mL. Further, O. indicum extract caused reduction of cell viability and induction of MCF-7 cells apoptosis along with induced ROS formation and increased caspase 3 activity. Moreover, the extracts caused the inhibition migration accompanied with reduced MMP 9 protein expression and decreased MMP 9 and ICAMP1 gene expression. Furthermore, O. indicum extracts strongly decreased expression of the cell cycle regulatory protein Rac1. In conclusion, O. indicum extract inhibits breast cancer cells viability and reduces cell migration. It could also be valuable for augmenting the activity of chemotherapeutic drugs used to treat breast cancer.