Kirtika Madan
Department of Pharmaceutical Science, Banasthali University, Jaipur, Rajasthan, India
Title: Amelioration of chemically induced nociception & inflammation by 7-hydroxy coumarin through the modulation of COX-2, iNOS and inflammatory cytokines
Biography
Biography: Kirtika Madan
Abstract
Aim: The objective of the current investigation was to explore the analgesic effect of naturally occurring coumarin and the involvement of inducible cyclooxygenase (COX-2), inducible nitric oxide synthase (iNOS) and cytokines in the observed effect.
Materials and methods: Acute toxicity of 7-hydroxy coumarin was evaluated according to OECD guidelines. Anti-nociceptive effect was explored by inducing chemical hyperalgesia using acetic acid and formalin in mice. ED50 of 7-hydroxy coumarins was calculated in acetic acid model. Modulation of cyclooxygenase and nitric oxide pathway by 7-hydroxy coumarin was examined by stimulator/precursor challenge with substance P and L-arginine respectively and quantification of COX-2 and iNOS expression by immunohistochemical analysis in spinal tissues. Involvement of inflammatory cytokines TNF-α and IL-1β was investigated using LPS challenge and subsequent ELISA analysis of these inflammatory mediators in serum. Carrageenan inflicted paw edema was employed to explore the anti-inflammatory activity of 7-hydroxy coumarin.