Hiwa K. Saaed
University of Sulaimani KRG-Iraq
Title: QRT-AAGEL-Quantitative Real Time PCR Analysis of Apoptotic Gene Expression In Chronic Lymphocytic Leukemia Patients and Their Relationships With Chemosensitivity
Biography
Biography: Hiwa K. Saaed
Abstract
To determine the role of Bcl-2 and p53 apoptosis related genes expressions in chronic lymphocytic leukaemia (CLL) patients concerning the response to different chemotherapy regimens and number of treatment courses. The study conducted on 55 CLL patients (44 CLL and 11 CLL/SLL; small lymphocytic leukaemia) and forty healthy individuals as control, at Hiwa Hospital of oncology & haematology in Sulaimaniyah during three-month period, October 1st- Dec 13th 2013. The RNA was extracted by exploitation total RNA extraction kit, treated with DNAse, then cDNA was synthesized and qRT-PCR used to analyse anti-apoptotic Bcl-2 and tumour suppresser p53 gene expressions. CLL/SLL showed higher Bcl-2 and p53 gene expression than CLL. CLL patients showed one-third increase in Bcl-2 gene expression compared to healthy controls (p<0.05), and one-half decrease in p53 gene expressions (p<0.05). Bcl-2 gene expression was higher, particularly, for those who were treated with higher range of treatment courses and fludarabine cyclophosphamide rituximab (FCR) regimen. P53 gene expression reciprocally related with Bcl-2 and vice versa. In conclusion, the results of this study indicated that both of the anti-apoptotic Bcl-2 family members and additionally the tumour suppresser p53 may be thought-about as a result of the key choices of cancer and significantly contributes to the impact of current treatment modalities on cancer cells. Moreover, a marked role for p53 gene in the chlorambucil resistance in CLL is elucidated.