Atmani-Kilani Dina
University of Bejaia, Algeria
Title: Hepatoprotective activity of the ethanolic extract of C. flammula against paracetamol toxicity in mice
Biography
Biography: Atmani-Kilani Dina
Abstract
Paracetamol is widely used to treat pain and fever. However, it creates oxidative stress by causing the depletion of glutathion which leads to hepatic lesions and necrosis Clematis flammula is a plant widely used in folklore medicine in Algeria to treat inflammatory diseases. The hepatoprotective activity of this plant extracts were evaluated in vivo against paracetamol toxicity by the assessment of MDA levels superoxide dismutase and catalase activities. Histopathological analysis was equally carried out to confirm our results. Results demonstrated an increase in MDA levels (6.61±0.75 nmol/mg prot) and a decrease in the activities of superoxide dismutase (3.557±0.91 U/mg protein), catalase (2.41±0.49 U/mg protein), glutathione (2.45± 0.60 μmol/mg de protéine) in paracetamol (400mg/kg)-treated group (positive control), compared to negative control. Clematis flammula plant extracts, on the other hand, contributed at reducing MDA levels (2.34±0.47) and increasing the activities of superoxide dismutase (6.068±0.85 U/mmg protein), glutathion (17.80±2,42 μmol/mg proteins) and catalase (10.64±2,01 U/mg protein) at 100 mg/kg. Histopathological analysis confirmed the hepatoprotective activity of this plant against paracetamol toxicity.