Irmak Dik
University of Selcuk, Turkey
Title: Effects of inactive parapoxvirus ovis on cytokine levels in rats
Biography
Biography: Irmak Dik
Abstract
Inactive parapoxvirusovis (iPPOV) is a commercial product widely used in veterinary medicine as an immune stimulator and/or non-specific paraimmune activator for protection against various infectious diseases. The aim of this study is to determine the effects of iPPOV on pro-inflammatory and anti-inflammatory cytokine levels in rats. A total of 56 rats were used in the present study. iPPOV (1 mL/rat) was administered intra peritoneal route to all rats, except for 7 rats (Control, 0 group). Serum samples were collected from 7 rats at the 1st, 2nd, 4th, 8th, 12th, 16th and 24th hours after treatments. Serum levels of Tumor Necrosis Factor-alpha (TNF-α), Interleukin (IL)-6, IL-12 and IL-10 were determined using commercially available Enzyme Linked Immunosorbent Assay kits. Administration of iPPOV stimulated TNFα (16th and 24th hours) and IL-6 (12th, 16th and 24th hours) synthesis and caused fluctuations in IL-10 and IL-12 concentrations. In conclusion, increased cytokine levels could be attributed to immunomodulatory activity of iPPOV, however, detailed studies are required to fully understand effects of iPPOV on immune system.
