Djebbar Atmani
University of Bejaia, Algeria
Title: Effect of Fraxinus angustifolia (Oleacea) leaf and bark extracts on acute and chronic inflammation: enhanced activity of nanovesicle-trapped extracts
Biography
Biography: Djebbar Atmani
Abstract
In the present study, hydro alcoholic extracts of leaves and barks of Fraxinus angustifolia, a medicinal plant widely used as anti-inflammatory in folk Algerian medicine were studied using several in vitro models and on acute and chronic inflammation. In the carrageenan-induced mouse paw edema test, barks and leaves extracts (150 mg/kg) showed a marked inhibitory effect on edema. Furthermore, both extracts significantly reduced edema (66% and 81%, respectively) and subchronic inflammation (47 and 51%, respectively) induced by 12-O-tetradecanoylphorbol 13-acetate (TPA) (1 mg/ear). In addition, both extracts inhibited the activity of the pro-inflammatory enzyme, myeloperoxidase (MPO) (54% and 69%, respectively) and NO production (78 and 94%, respectively) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, with no apparent toxicity. Moreover, the extracts demonstrated a considerable hypo-uricemic effect in mice, with an appreciable inhibition of xanthine oxidase activity. F. angustifolia leaf and bark extracts trapped in Nano vesicles significantly enhanced healing of TPA-induced skin lesions in mice, protected human keratinocytes against H2O2-induced oxidative damage and exhibited an outstanding antioxidant activity against DPPH and ABTS. The presence of quercetin, catechin, rutin and tannic acid in F. angustifolia extracts may account for the observed activities which justify the use of this plant in traditional medicine against pathologies associated with oxidative stress and inflammation
