Day 1 :
Keynote Forum
Dr. Anka G. Ehrhardt
Bristol-Myers Squibb Company, USA
Keynote: Velvet Revolution in Biomarkers: Transforming and Scaling the Art of Flow Cytometry for the Omics Age
Time : 9:50-10:30
Biography:
Dr. Anka G. Ehrhardt is a biophysicist with a doctorate degree in human physiology. She is currently working in the United States building and directing a team applying the latest technologies in support of the development of new live saving drugs
Abstract:
A major part of modern biomarker research relies on –omics data, the systematic collection and analysis of big data for various biological patterns, most notably genomics and proteomics. At the same time, with the current intense focus on immunooncology, former niche-technologies are becoming key read-outs for biomarker studies, notably flow-cytometry based immunophenotyping with its capability to characterize the immune-status of patients. Given the complexity of cell populations in the immune-system, an –omics approach seems desirable to find various biomarkers, e.g. for patient stratification, target discovery or rational design of combination therapies. However, in its current state, flow cytometry is as much an art as a science, and does not provide sufficient reproducibility and data comparability for the creation of large data sets. Dr. Ehrhardt’s presentation shows how a rigorous focus on problem solving innovation and quality allows the creation of consistent large databases of cytometry-based immunophenotyping information, and key strategies to mine the collected information for the urgently needed new biomarker information.
Keynote Forum
Anna Szemik-Hojniak
University of Wroclaw, Poland
Keynote: Favorable for Drug Candidates the CT Properties of Alkylamino Pyridine N-oxides in Solution
Time : 10:30-11:10
Biography:
Anna Szemik-Hojniak has completed her PhD in Radiochemistry from the University of Wroclaw and postdoctoral studies in Physical Chemistry and Radiochemistry from Solvay Foundation (Belgium), KU Leuven (Belgium) and Centre of Nuclear Researches (Strasburg, France). She occupies with Organic Molecular Photo physics, published more than 45 papers and serves as a reviewer in scientific journals. In the years (2002-2011) she served as the INWES Board director (2002-2011) for INWES corporation- the operational partner of UNESCO. In 2007, at the University of Wroclaw, (Poland), she organized international workshop “Strategies for the Highly Skilled Global Workforce”. In 2011, dr Szemik was awarded with Distinguished Service Award. Presently she is INWES-ERI General Secretary and the Board Director (headquarters-Ottawa, Canada).In 2014 and 2015; she organized Mini Symposium “Photo physics of Electron and Proton Transfer” in the framework of ICCMSE- 2014 conference, in Athens (Greece) and presented the Keynote lecture on “Behaviour of styryl derivatives under the light irradiation” during 4th-Annual European Pharma Congress-2015 in Valencia (Spain).
Abstract:
The spectrum of therapeutic activities of heterocyclic N-oxides and specifically those of pyridine N-oxides is rather broad. Since their discovery about twenty years ago, they were broadly recognized as antibacterial, antihypertensive, anti-inflammatory, ant parasitic, as well as anti-HIV, anti-inflammatory, and neuroprotective agents. The heterocyclic N-oxides form a new class of bio reductive drugs with favourable drug-receptor interactions of the charge transfer (CT) type. Different N-oxides have been detected in animals, plants, and microorganisms, and therefore it is an utmost important issue to recognize their physico-chemical and physiological properties. The former (CT) interactions in N-oxides are strongly enhanced under the light irradiation and the excited electronic CT state results from electron transfer (ET) between electron donor /acceptor (electron-donating/withdrawing) functional groups. When additionally, the N-oxide contains the hydrogen donor and hydrogen acceptor substituents, the compound enhances its reactivity and may exhibit also the proton transfer process that may occur as a concerted or sequential combined Proton Coupled Electron Transfer (PCET) process. The PCET is pivotal both in biological systems and in bioenergetics conversion. Example of the former is the reduction of protons to hydrogen by hydrogenase and for the latter is the energy storage process.rnrnFig.1. Molecular structure of alkylamino nitro pyridine N-oxides rnrnA series of new alkylamino- (NHR-) type hydrogen bonding compounds including 2-aminobuthyl and 2-aminomethyl (4 nitro)-5-or 6 methyl pyridine N-oxides (see Fig.1) were designed and synthesized. This makes feasible the comprehensive spectroscopy and dynamics studies of amino-nitro excited state electron transfer (ET) and excited-state intramolecular proton transfer (ESIPT) along the N-H…O hydrogen bonding in the systems under investigations. In this talk, the PCET process in the electronic excited state of targeted pyridine N-oxides in solution where the normal HB form and the PT form exhibit the CT nature will be discussed both from experimental (absorption, steady state and time-resolved fluorescence) and theoretical [TD DFT B3LYP/6-31G(d,p) calculations] point of view.
Keynote Forum
Hulya Ozdemir
Yuzuncu Yil University, Turkey
Keynote: The effect of Dieatary Boran o learning and behaviour in rats given Boric acid
Time : 11:35-12:15
Biography:
Hulya Ozdemir has completed her PhD in Faculty of Medicine; she is working as a Professor at the Medicine Faculty of Yuzuncu Yıl University. She’s the head of department of Pharmacology and Toxicology. She has published more than 70 papers and her research areas are mainly with plant therapy on the diabetes and cancer diseases, antioxidants and behavioural pharmacology.
Abstract:
This study was designed to investigate the effect of dietary boron on spatial learning, anxiety, some vitamin and oxidative parameter levels in rats given boric acid. Thirty two Wistar male rats average weight of 200 ± 20 g were used in this study. Rats were divided in four equal groups (n=8), control group fed with a standard rodent diet and experimental groups fed with 250, 500 and 1000 ppm boric acid enriched diet (equivalent to 4.1, 8.2 and 15.0 mg B/kg body weight). In a five-week long study the Elevated plus-maze was used for anxiety assessment and Morris water maze for evaluation of spatial learning. Additionally, blood samples were obtained at the end of the study and used to determine some vitamin and oxidative parameter levels. The dietary boron significantly increased weight gain (p<0.001) and food consumption in 250 and 500 ppm BA group (p<0.05). Although it did not affect learning process or anxiety related behaviour significantly, in 1000 ppm BA group it showed positive effects over memory consolidation (p<0.05). Biochemical analyses showed significant decrease of MDA (p<0.05) and increase of D3 vitamin levels (p<0.01) in 500 ppm BA group, significant increase in GSH-Px activity in 250 andrn500 ppm BA group (p<0.05) whereas vitamin E decreased in all groups (p<0.05). Total serum antioxidant capacity and retinol levels of experimental groups were not found significantly different. As a result, our study demonstrate that dietary boron in given doses has positive effects on performance of rats, memory consolidation, lipid peroxidation, glutathione peroxidase activity and vitamin D3 levels and, therefore, can be beneficial for health if used in right dosage notes: This article is written from a master‘s thesis of Bilyana Yaren and This research was supported by the Research Fund of the University of Centenary Year as Project No. 2010-SBE-YL137.rn
Keynote Forum
Yahdiana Harahap
Universitas Indonesia, Indonesia
Keynote: Dried Blood Spot Sampling in Combination with LC-MS/MS for Clinical Pharmacokinetic Study and Therapeutic Drug Monitoring
Time : 12:15-12:55
Biography:
Yahdiana harahap has completed her PhD at the age of 39 from Department of Pharmacy, Institute Technology Bandung, Indonesia. Now she is the Head of Bioavailability and Bioequivalence laboratory Faculty of Pharmacy, Universitas Indonesia. Prior to this position, she was the Dean of Faculty of Pharmacy, Universitas Indonesia. She has published 50 papers published in both International and National Journals. She has been invited to be the speakers in many international conference, especially in the field of BA/BE and Bioanalaytical technique. She currently serves as an expert at Indonesia National Agency of Drug and Food Control, specifically in BA/BE evaluation.
Abstract:
Plasma has been the mainstay matrix for measurement of systemic concentration of compounds used in the assessment and evaluation of pharmacokinetics and therapeutic drug monitoring. As an alternative, dried blood spot (DBS) is an innovative bio-sampling method in which a blood sample is collected on wet filter paper and then was dried 2-3 hours at room temperature or under the flow of nitrogen gas with controlled humidity. Bio sampling techniques using dried blood spot samples method have advantages such as; minimum invasive because of using a sterile lancet needles in the fingers, toes or heel, only requires small sample volume, cost-effective of storage and distribution, the analytes are stable thus reduces the risk of infection. Dried blood sample method can help the application of pharmacokinetics and toxicokinetics test, drug monitoring, disease screening, and doping test. It has been applied in the pharmaceutical industry, hospitals and research centres, especially for samples with small volume and difficulty in collecting, storage, process and transportation. On the other hand the use of whole blood as a sample taken from the periphery with small volume can cause very low level of analyte to be analysed and the hematocrit would greatly interfere the quantitative analysis of the drug molecule. The use of liquid chromatography with tandem mass spectrometry (LC-MS / MS) combined with optimum extraction procedures can be a competitive solution to sensitively analysing small drug molecule from dried blood samples method for pharmacokinetics study and therapeutic drug monitoring.
- Pharmacognosy & Phytochemistry | Novel Drug Delivery System | Pre-formulation Studies
Chair
Rajarajeshwary N
Rajiv Gandhi University of Health Science’s, India
Co-Chair
Namita Naik Khanvte
Rajiv Gandhi University of Health Science’s, India
Session Introduction
Pierre Lutgen
IFBV-BELHERB, Luxembourg
Title: Artemisia plants are deadly weapons against malaria
Biography:
Dr Pierre Lutgen was born 1940 in Luxembourg, studied at the University of Louvain in Belgium and obtained diplomas in chemistry, social sciences and philosophy. He worked during 25 years for the Dupont CY, in research, and during 8 years in the steel industry, mainly in the environmental field. Since his retirement he worked as consultant for health and environment as invited professor at the University in Medellin and for the European Communities in several countries. Over the last ten years he has organized the association IFBV-BELHERB studying and fighting tropical diseases with some 30 academic and medical partners in Africa, South America and Europe. Numerous peer reviewed papers have been published by this team, mainly on herbal medicine.
Abstract:
Since 7 years the association IFBV-BELHERB from Luxembourg has established a working relationship with African and South American universities, in close cooperation with other European research institutions. Several of these partners have run clinical trials with Artemisia annua team. In all these trials a therapeutical effect of 95 % or higher was confirmed by the use over 7 days of whole leaf infusion, capsules or tablets. One of the surprising effects noticed in these trials was that that the artemisinin content had very little impact on the results. This lead us to make an analysis as complete as possible of all the constituents, organic and inorganic, in a large series of A. annua samples from different origins. A. annua from Luxembourg which had shown very promising antimalarial results, excellent bactericidal properties and a strong anti-inflammatory effect contained very little artemisinin but higher concentrations of certain essential oils. The effect of water soluble polysaccharides, phytosterols and saponins has been neglected in the past because most of the A. annua extracts had been obtained with organic solvents. Several papers have shown that A. annua ingested as powdered leaves or in conjunction with fatty food significantly increases the artemisinin concentration in the blood. It is well documented in the literature that A. afra or sieberi which contain little or no artemisinin are extensively used as antimalarials. They contain at least 5 molecules of the same antimalarial efficacy as artemisinin. At Leiden it was found that the anti-HIV activity of A. afra is even higher than for A. annua. More recent research from the Al Quds University has shown that aqueous infusions of several Artemisia species strongly inhibit beta-hematin, like chloroquine did. But the most important finding in several of the clinical trials, especially in Kenya and Uganda, was that people who drink one or two cups of A. annua tea per week become immune against malaria. Similar strong prophylactic results have been obtained with ARTAVOL, a mixture of herbs developed by the Ministry of Health in Uganda, mixture containing Artemisia without artemisinin. Resistance in this case is not related to the killing power of one single molecule like artemisinin but to the polytherapy of the whole plant which not only eliminates the parasites but boosts the immune system, avoiding thus infection, reinfection or recrudescence. Recent clinical trials in Senegal and RD Congo also have shown that Artemisia inusions are more efficient against Schistosomiasis mansoni than Praziquantel
Djebbar Atmani
University of Bejaia, Algeria
Title: Antidiabetic, anti-lipoperoxidation and hepatoprotective activities of Fraxinus angustifolia and Pistacia lentiscus extracts
Time : 13:15-13:35
Biography:
Professor Djebbar Atmani has completed his Master of Science degree at California State University, Los Angeles (USA) in 1987 and his PhD at the University of Sétif (Algeria) in 2004. He is the acting Dean of the Faculty of Nature and Life Sciences, University of Bejaia (Algeria). He has published more than 15 papers in reputed journals and has been serving as reviewer of many journals.
Abstract:
Fraxinus angustifolia (Lamiaceae) and Pistacia lentiscus L. (Anacardiaceae) are two common Mediterranean plants used extensively in Algerian traditional medicine to cure inflammatory-related disorders as well as symptoms of diabetes. The anti-diabetic effect of F. angustifolia (leaves and bark) and P. lentiscus (leaves and fruits) extracts was evaluated using the streptozotocin-induced diabetes model in rats, whereas anti-lipoperoxidation activity was assessed with the malonaldehyde assay after injection of paracetamol to mice. The results indicate that F. angustifolia leaf extract (50 mg/Kg of body weight) and P. lentiscus leaf extract (100 mg/kg of body weight) induced a significant reduction (60%) of blood glucose level after 2 hours of administration of the extracts. Furthermore, extracts from both plants significantly reduced α-amylase activity. Analysis of liver homogenates showed an important decrease in the concentration of malonaldehydes in the group of mice treated with plant extracts (0.25 ± 0.006 and 0.26 ± 0.03 for F. angustifolia and P. lentiscus extracts, respectively), compared to that of non-treated animals (0,45± 0.005). Furthermore, plant extracts tested with concentrations up to 200mg/kg of body weight showed no signs of toxicity. Moreover, histological examination of the liver revealed significant protection against paracetamol-induced hepatic necrosis. Phenolic compounds identified in plant extracts could take, at least, partial responsibility for the observed anti-hyperglycaemic and antioxidant activities, thereby conferring these plants a promising protective capacity against oxidative stress.
Shirly Kumala
Pancasila University, Jakarta
Title: Antimicrobial activity and Identification of Chemical Compounds of Secondary Metabolites Bioproduction of Endophytic Fungi from Jamblang Plant (Eugenia cumini L.)
Time : 14:05:14-25
Biography:
Shirly Kumala has completed her PhD from Biomedik Faculty of Medical, University of Indonesia, Jakarta. She is the Dean of Faculty Pharmacy. Pancasila University Jakarta, Indonesia. She has published more than 25 papers in accredited journals and has been serving as a reviewer in journal of Pharmacy.
Abstract:
Colletotrichium capsici endophytic fungi isolated from Jamblang plant (Eugenia cumini L.). Endophytic microbes were microbes that spent part or all of their life within plant tissue or of a host plant. They produce secondary metabolites with potent anti-microbial activity. Jamblang (Eugenia cumini L.) is one of the traditional medicinal plants that grow in Indonesia and were known by the layman as a plant that has efficacy as anti-diarrheal and anti-diabetic.This research focussed on isolation of the endophytic microbes from branches of Jamblang plants and their secondary metabolites. Isolation of endophytes were performed in PDA(potato dextrose agar) using direct seed plant. Endophytic fungi isolates that have the greatest antimicrobial activity against the bacteria Staphylococcus aureus, Escherichia coli, and then fermented in Potato Dextrose Yeast (PDY) in large scale. Supernatant was extracted with ethyl acetate solvent.. Ethyl acetate extract fractionated by column chromatography (SiO3, n-hexane- ethyl acetate = 50:1 ~ 1:1) and obtained three fractions. Further, agar diffusion method was performed to assess their anti-microbial activity Antibacterial test results indicate the fraction III had the antibacterial activity against Staphylococcus aureus with the inhibition diameter of 10.7 mm and but no antibacterial activity against Escherichia coli. Identification of chemical compounds by GC-MS showed that the fraction III is dominated by compound fatty acids and phenolic groups. In conclusion, secondary endohpytes isolated from Jamblang plants branches contained predominantly fatty acid and phenol related compounds that could be responsible for its potent anti-microbial activity.
I.Ermolina
De Montfort University School of Pharmacy, Leicester, UK
Title: Physicochemical and pharmaceutical properties of HEA-HEMA hydrogels
Biography:
Irina Ermolina has completed her PhD from Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences in 1995, following by postdoctoral studies from Hebrew University of Jerusalem, Glasgow University and South Hampton University. Currently, she is a senior lecturer at De Montfort University, Leicester, UK, theaching the pharmaceutical technology, pharmaceutical material sciences and analytical techniques. She has published 45 papers in the peer reviewed journals and has been serving as a reviewer for several sceintific Journals.
Abstract:
Over the last years the hydrogels have attracted considerable attention due to their capability to be used for numerous pharmaceutical and biomedical applications (i.e. drug delivery systems, contact lenses and wound dressings). Hydrogels are swell able polymeric materials capable of imbibing a large amount of liquid and, therefore, a large amount of drug. Recently we have described novel cross-linked 2-hydroxyethylacrylate-co-2-hydroxyethylmethacrylate (HEA-HEMA) hydrogels with different co-polymer compositions synthesized by three-dimensional free-radical copolymerisation. The aim of the present work is to characterize the physicochemical and pharmaceutical properties of HEA-HEMA hydrogels, which potentially can be used as sustain drug delivery systems. The effects of the hydrogel composition (HEA/HEMA ratios) and liquid medium (aqueous PEG and glycerol solutions) on morphology, swelling and mechanical properties were examined using different analytical techniques. The molecular dynamics results for hydrogels were obtained by Broadband Dielectric Spectroscopy. The current study has been specifically focused on the analysis of the free/bound water redistribution in the hydrogels using thermo analytical techniques. Also the effect of different methods of sterilization was studied with respect to the stability of hydrogels. The pharmaceutical aspect of this study included the analysis of drug loading and release for the cases of low molecular weight drugs (riboflavin, ibuprofen) and proteins (lysozyme). The results show that HEA-HEMA hydrogels can be used as a sustain drug delivery system for both small drugs and bio macromolecules. The variation of the HEA-HEMA composition as well as liquid medium permits controlling the properties of hydrogels allowing producing the hydrogels with the drug release rate desired.
Atmani-Kilani Dina
University of Bejaia, Algeria
Title: Hepatoprotective activity of the ethanolic extract of C. flammula against paracetamol toxicity in mice
Time : 14:45-15:05
Biography:
Atmani-Kilani Dina has completed her master degree from California State University, Los Angeles and her PhD from University of Bejaia in Algeria and postdoctoral studies from University of Bejaia. She is the director of a project on the biological activities of Clematis flammula and is involved in two other projects on the biological activities of other local plants in Algeria. She has published more than 7 papers in reputed journals and is pursuing her research in order to identify many novel molecules in C. flammula with the collaboration of Dr. Tristan Richard in Bordeaux France and José Ignacio Ruiz Sanz in the University of the Basque Country in Bilbaoa
Abstract:
Paracetamol is widely used to treat pain and fever. However, it creates oxidative stress by causing the depletion of glutathion which leads to hepatic lesions and necrosis Clematis flammula is a plant widely used in folklore medicine in Algeria to treat inflammatory diseases. The hepatoprotective activity of this plant extracts were evaluated in vivo against paracetamol toxicity by the assessment of MDA levels superoxide dismutase and catalase activities. Histopathological analysis was equally carried out to confirm our results. Results demonstrated an increase in MDA levels (6.61±0.75 nmol/mg prot) and a decrease in the activities of superoxide dismutase (3.557±0.91 U/mg protein), catalase (2.41±0.49 U/mg protein), glutathione (2.45± 0.60 μmol/mg de protéine) in paracetamol (400mg/kg)-treated group (positive control), compared to negative control. Clematis flammula plant extracts, on the other hand, contributed at reducing MDA levels (2.34±0.47) and increasing the activities of superoxide dismutase (6.068±0.85 U/mmg protein), glutathion (17.80±2,42 μmol/mg proteins) and catalase (10.64±2,01 U/mg protein) at 100 mg/kg. Histopathological analysis confirmed the hepatoprotective activity of this plant against paracetamol toxicity.
- Genetics & Genetic Engineering | Nanotechnology | Bio-Pharmaceutics | Hospital Pharmacy
Chair
Arik Dahen
Ben-Gurion University of the Negev, Israel
Co-Chair
Adalberto Rezende Santos
Oswaldo Cruz Institute – Brazil
Session Introduction
Hulya Ozdemir
Yuzuncu Yil University, Turkey
Title: The effect of resveratrol therapy on serum total sialic acid and lipid-bound sialic acid in male rats with chronic fluorosis
Time : 15:05-15:25
Biography:
Hulya Ozdemir has completed her PhD in Faculty of Medicine; she is working as a Professor at the Medicine Faculty of Yuzuncu Yıl University. She’s the head of department of Pharmacology and Toxicology. She has published more than 70 papers and her research areas are mainly with plant therapy on the diabetes and cancer diseases, antioxidants and behavioural pharmacology.
Abstract:
Fluor is strongest electronegative element, a potent anion and cumulative toxin. This study was designed to evaluate the effect of resveratrol on serum total sialic acid (TSA) and lipid bound sialic acid in the rats chronically exposed to fluoride. The study was administered using 32 male Sprague Dawley rats weighing 200-250 g. Rats were divided into four groups (n=8/group). Group I comprised the control group, group II was treated with NaF (10 mg/lt/day), group 3 was treated resveratrol (50 mg/lt/day) and group IV was treated NaF+resveratrol for 90 days period. Total sialic acid (TSA) and lipid-bound sialic acid (LSA) were determined using spectrophotometric method. As a result of the analysis, It was seemed that LSA level compared with the control group increased in NaF group (p<0.05). On the other hand the resveratrol group was also significantly lower than the NaF group regarding LSA and TSA levels (p<0.05 and p<0.01 respectively). Whereas, the resveratrol + NaF group was significantly higher than the resveratrol group regarding TSA levels (p<0.05). Results from this study suggest that resveratrol may be partially effective in preventing the negative effects of fluorosis in male rats.
Adalberto Rezende Santos
Oswaldo Cruz Institute –Brazil
Title: Pharmacogenetics research in different disease models: genetic diversity in an ethnic admixture population
Time : 15:45-16:05
Biography:
Adalberto Rezende Santos is Ph.D in Cellular and Molecular Biology from Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. Actually, he is a Senior Investigator and Head of the Laboratory of Molecular Biology Applied to Mycobacteria of Oswaldo Cruz Institute, Supervisor at the post-graduation programs of Cellular, Molecular Biology, and Clinical Medicine from Fiocruz and Federal University of Rio de Janeiro respectively. He is ad hoc consultant of the Executive Secretariat of Science Technology and Environment and of the Ministry of Health, Brazil. He is also referee of the Journal of Infectious Diseases and Pharmacogenomics.
Abstract:
In humans, the enzyme N-acetyltransferase2 (NAT2), coded by NAT2 gene, is the main metabolizer of isoniazid, dapsone and hydralazine, used for the treatment of tuberculosis, leprosy and resistant hypertension, highly incident diseases in Brazil. We studied NAT2 in different Brazilian populations for these three diseases. In the first study, we showed the predominance of NAT2 slow acetylation alleles in Rio and Goias states. However, population from Rio showed a higher heterogeneity in NAT2 allele distribution and significant higher frequency of intermediate phenotype. We identified six new SNPs allowing characterization of seven new alleles. Further, we performed an in silico molecular modelling and structural protein analyses of NAT2. The results strongly indicates the direct involvement of the new SNP (152G>T-Gly51Val) in substrate recognition, SNP (203G>A-Cys68Tyr) in the modification of the catalytic site by the loss of a functional group and SNPs (458C>T, 578C>T, 683C>T and 838G>A) in enzyme degradation, all altering the acetylation activity to slow acetylation. In a subsequent study to evaluate the influence of CYP2E1, GSTT1, GSTM1 and NAT2 genotypes on isoniazid-induced hepatitis in TB patients, we found that only the NAT2 slow acetylation phenotype represented a risk factor for the occurrence of this outcome during TB treatment. In a more recent study, the influence of the acetylation phenotypes in anti-hypertensive effect of hydralazine in patients with RH was evaluated. Again, the predominance of slow acetylation phenotype was observed and only slow acetylators had significant blood pressure reductions after hydralazine use, however, with a high incidence of ADRs.
Ronny Martien
Gadjah Mada University, Indonesia
Title: Formulation Insulin Nanoparticle Using Chitosan and Pectin Polymers and their pharmacokinetic profile with oral application
Time : 16:05-16:20
Biography:
Ronny Martien has completed his PhD at the age of 30 years from Innsbruck University Austria (2007) and postdoctoral studies from Univ. Innsbruck (2010). He is the head of research group, BINDR (Biopolymer and Nano Delivery Research). He is a head of PhD Program at Faculty Pharmacy, Gadjah Mada University, Indonesia. He has published more than 10 papers in reputed journals and has been serving as an editorial board member of repute. His research topic are nano particulated drug delivery system, expecially with biopolymers as a matrix.
Abstract:
Parenteral insulin is one of the major therapeutic for patients with Diabetes Mellitus (DM) especially in Type 1, which often leads to patient discomfort and other problems due to the use of it within a certain period. Formulate insulin in oral dosage form is the main choice to resolve the issue. However, application of insulin orally encounters obstacles such as degradation by protease enzyme and poor insulin permeability of the gastrointestinal tract. One solution offered is insulin formulation into the nanoparticles form. In this study, nanoparticle formulation of insulin will use a combination of low molecular weight chitosan and pectin that serves to protect insulin from degradation and also enhance insulin absorption through the gastrointestinal mucosa. The aim of this study was to obtain the optimum formula of insulin nanoparticles that can be used as an alternative therapy for patient with DM. Preparation of insulin nanoparticles carried by ionic gelation method utilizing polyelectrolyte interaction between (-NH3+) of chitosan and (-COO-) of pectin to form nanoparticles that are compact and stable charge. Formula optimization was performed using Factorial Design 22 with Design Expert® 7.1.5 software. Concentration and pH of the pectin were used as factors, while the entrapment efficiency, particle size and polydispersity index were used as responses. The optimum formulas were further evaluated like zeta potential, particle morphology, profile spectra of FT-IR and in vitro release study. The obtained optimum formula consist of chitosan of 0.05% and 0.4% pectin (pH 5.0) with the mean entrapment efficiency of 63,59% ± 2,17, particle size of 228,3 nm ± 26,3, polydispersity index of 0,354 ± 0,042, zeta potential 49,40 mV ± 11,59, particle round and dark, polymer complexation was confirmed by FT-IR and release profile following the kinetics Korsmeyer-Peppas models with non-Fickian release mechanism on media HCl buffer pH 1.2 (n = 0.454) and Fickian release mechanism on PBS buffer pH 6.8 (n = 0.369). Nanoparticle insulin have AUC [(114,9917 ± 11,88) ng.menit/mL] higher than unmodified insulin [AUC = (67,8830 ± 2,17) ng.menit/mL] and PBS as negative control [AUC = (62,9713 ± 9,12) ng.menit/mL] with p < 0,05. Conclution from these results are insulin nanoparticle significantly improved insulin concentration in the blood serum higher than unmodified insulin per oral.
Emad B. Basalious
Cairo University, Egypt
Title: Self-nanoemulsifying self-nanosuspension (SNESNS) for enhancing oral bioavailability of diacerein: Simultaneous portal blood absorption and lymphatic delivery
Time : 16:25-16:45
Biography:
Emad B. Basalious has completed his PhD and postdoctoral studies from Faculty of Pharmacy, Cairo University. He is an associate professor in department of pharmaceutics and Industrial pharmacy, Cairo University. He has published more than 20 papers in reputed journals. He is working as R&D technical consultant for several Egyptian pharmaceutical companies.
Abstract:
The application of self-Nano emulsified drug delivery system (SNEDDS) to improve bioavailability of diacerein (D) has been hampered by its large dose and limited solubility. This work aimed to prepare diacerein loaded self-Nano emulsifying self-Nano suspension (D-SNESNS) containing high drug load. DSNESNS was prepared by homogenizing D into MaisineTM-based SNEDDS that gave the highest drug solubility. D-SNESNS was evaluated for particle size, zeta potential and in vitro dissolution. Significant increase of D solubility was observed from D-SNESNS ( 309 mg/mL) than traditional SNEDDS (162 mg/mL) due to the spontaneous simultaneous formation of Nanoemulsion and Nano suspension (top–down approach). When exposed to water with mild agitation, the drug micro particles in D-SNESNS are temporarily surrounded by unsaturated aqueous layer (containing optimum concentrations of surfactant and co-solvent) that facilitates the erosion of the suspended drug particles into Nano sized ones. Nanoemulsion-based nano suspension (NENS) was confirmed using transmission electron microscopy and particle size analysis. D-SNESNS equivalent to 50 mg D exhibited complete and very rapid dissolution after 15 min in phosphate buffer pH 6.8 due to the existence of D as solubilized molecules inside Nanoemulsion globules and Nano sized suspended drug particles forming D-NENS. The relative bio availabilities of rhein from D-SNESNS in rats with normal and blocked chylomicron flow were about 210% and 164%, respectively in comparison to aqueous D suspension. The significant increase in the dissolution, portal absorption and lymphatic delivery of D propose that SNESNS could be promising to improve oral bioavailability of poorly water soluble drugs that have limited drug load in SNEDDS.
Christina Yuen Ki Leung
The University of Hong Kong – Shenzhen Hospital (HKU-SZH), Shenzhen, China
Title: Advanced Clinical Pharmacy System in a Reformed Hospital in China aiming to improve the quality of patient care and to enhance the job satisfaction level for the clinical pharmacists
Time : 16:25-16:45
Biography:
Christina Leung completed two Bachelor degrees in England, Management Sciences degree followed by a Pharmacy degree. Following the registration as a pharmacist in England, she worked in a number of teaching hospitals in London. After the completion of junior pharmacist training, Ms Leung spent 12 years as Women’s and Children’s Pharmacist, mainly specialising in Paediatric ICU, Paediatric Liver, Obstetrics and Gynaecology. She published a number of articles including two articles relating to drugs use in paediatric liver diseases published in UK Healthcare magazine. Ms Leung is also a registered pharmacist in HK and she is currently working as the Senior Pharmacist (Clinical Pharmacy Service) at the HKU-SZH in China. She is also the Honorary Lecturer of the Department of Pharmacology and Pharmacy at the University of Hong Kong.
Abstract:
HKU-SZH adopts the good pharmacy practices from the West and has implemented an advanced clinical pharmacy system. We have been using near-patients and near-doctors approach to provide high quality of clinical pharmacy service to patients and healthcare professionals to ensure safety and efficacy of drugs use. The clinical pharmacists join the doctor-led ward rounds regularly on selected wards such as ICU and Medicines wards. For all newly admitted in-patients, the clinical pharmacists carry out medication reconciliation, and the information is recorded in the electronic prescribing system permanently. They also check the in-patient prescriptions for clinical appropriateness using approved and updated reference sources. In addition, pharmacists involve actively in the warfarin patient counselling service and the stroke clinical pathway for our in-patients. Since early 2015, clinical pharmacists have participated in the smoking cessation clinic, paediatric respiratory out-patient clinic, diabetic clinic to provide patient counselling services. In addition, clinical pharmacists deliver drugs-related talks for patients in the cardiac rehabilitation centre and on endocrine ward, and have prepared medication-related patient leaflets. Furthermore, clinical pharmacists give talks to patients in the Out-Patient Forum regarding drugs use for smoking cessation, drugs use in hepatitis B, safe use of insulin injection, effective use of inhalation devices, medication safety in children including use of oral syringe. All these quality improvement plans are to enhance medication safety and optimistation of drugs use. Clinical pharmacists in China find this experience rewarding and they gain lots of job safisfaction by noticing the positive impact on the quality of patient care.